mouse apolipoprotein e (apoe) elisa kit  (MyBiosource Biotechnology)

Journal: Journal of Neuroinflammation

Article Title: The protective PLCγ2-P522R variant mitigates Alzheimer’s disease-associated pathologies by enhancing beneficial microglial functions

doi: 10.1186/s12974-025-03387-6

Figure Lengend Snippet: PLCγ2-P522R variant does not increase the plaque-associated APOE or DAM signature in female APP/PS1 mice. A Analysis of total APOE and APOE areas (µm 2 ) within and surrounding (within 0–10- and 10–20-µm distances from the amyloid plaque outline) β-amyloid plaques revealed no differences between the APP/PS1xPLCγ2-P522R (A+/P ki/ki ) and APP/PS1 (A+/P wt/wt ) mice. A+/P wt/wt n = 5, A+/P ki/ki n = 6. Colored disks on the x-axis indicate the analyzed area overlapping (middle circle) and surrounding β-amyloid plaques. The scale bar in the representative immunofluorescence images is 50 μm. Unpaired samples t test. All the data are presented as the means ± SEMs. Each data point represents an individual mouse. B Heatmap of z scored vst-normalized expression of homeostatic as well as stage 1 and stage 2 disease-associated microglia (DAM) signature genes in the bulk temporo-occipital cortex and C ) CD11b+ microglia isolated from the whole brain tissue of 13-month-old wild-type (A-/P wt/wt ), A+/P wt/wt and A+/P ki/ki female mice. Cortex A-/P wt/wt n = 1, A+/P wt/wt n = 4, and A+/P ki/ki n = 4; CD11b+ A-/P wt/wt n = 7, A+/P wt/wt n = 4, and A+/P. ki/ki n = 4

Article Snippet: Soluble and insoluble APOE were measured in lysates using an Apolipoprotein E (APOE) Mouse ELISA Kit (#ELK2007, Gentaur) according to the manufacturer’s instructions.

Techniques: Variant Assay, Immunofluorescence, Expressing, Isolation

Journal: Molecular Neurodegeneration

Article Title: Alzheimer’s disease associated isoforms of human CD33 distinctively modulate microglial cell responses in 5XFAD mice

doi: 10.1186/s13024-024-00734-8

Figure Lengend Snippet: Altered Aβ plaque composition by hCD33 isoforms. a Representative confocal fluorescent images showing the different degrees of plaque compaction by co-staining for total Aβ and ThioS. A diffuse plaque without ThioS staining at the far left, a plaque with small ThioS core in the middle, and a highly compact plaque at the far right are presented. Scale bar = 20 µm ( b-e ). Quantification of Aβ deposits containing a ThioS. + core. Representative epifluorescent images of Aβ deposits in the dorsal subiculum of 5XFAD mice at ( b ) 4 and ( d ) 8 months, with co-staining with anti-Aβ antibody (white) and ThioS (blue). Scale bar = 50 µm. Quantification for pooled males (squares; n = 5 per genotype) and female (circles; n = 5/genotype) mice at ( c ) 4 and ( e ) 8 months. f Representative confocal fluorescent images of plaque composition in the dorsal subiculum of 8 months old CD33M and CD33m mice. Scale bar = 20 µm ( g , h ) Quantification of plaque composition (ratio of Aβ over ThioS levels) in the dorsal subiculum of 5XFAD mice at ( g ) 4 and ( h ) 8 months. A total of 300 plaques per mouse, from 5 males and 5 females, were quantified for each group. i Biochemical characterization of PK-sensitivity of insoluble Aβ 1-42 from 5XFAD mice. j Quantification of the percentage of insoluble Aβ 1-42 levels after PK digestion compared to not treated with PK. k Quantification of the ratio of ApoE to Aβ 1-42 in the insoluble fraction from 5XFAD mice ( n = 9, 10, and 9 mice of control, CD33M, and CD33m genotypes, respectively)

Article Snippet: ApoE levels were quantified in both the soluble and insoluble fractions using a Mouse Apolipoprotein E (APOE) ELISA Kit (Mybiosource).

Techniques: Staining, Control

Journal: Molecular Neurodegeneration

Article Title: Alzheimer’s disease associated isoforms of human CD33 distinctively modulate microglial cell responses in 5XFAD mice

doi: 10.1186/s13024-024-00734-8

Figure Lengend Snippet: Single-cell RNA sequencing reveals Ccl3 and Trem2 DAM enriched in the CD33m genotype. a Unsupervised and iterative machine-learning based clustering of 15,200 microglia ( Hexb + Fcrls + Tmem119 + Sall1 + ) and BAM ( Ms4a7 + Mrc1 + Lyve1 + Timd4 + ) collected from 5XFAD control, CD33M 5XFAD, CD33m 5XFAD, and control non-5XFAD mice. Microglia from three homeostatic (HM1-3), two transitioning (TM1-2), two RNA binding protein (RBM1-2) subpopulations along with the disease-enriched interferon responsive (IRM), myelin transcript enriched (MTEM), and disease associated (DAM) clusters. b-e UMAPs of individual samples showing ( b ) 5XFAD control, c CD33m 5XFAD, d CD33M 5XFAD, and e control non-5xFAD. f , g Separation of each cluster by ( f ) proportion of cells and ( g ) absolute number of cells belonging to each genotype. DAM, specifically Trem2 DAM, are enriched in the CD33m group and reduced in the CD33M group. h Differential gene expression per cluster used to define microglial subpopulations. HM are characterized by homeostatic genes ( P2ry12, Tmem119 ), RBM by genes related to RNA binding ( Son, Fus ), TM by a combination of homeostatic ( P2ry12, Tmem119 ), complement ( C1qa, C1qb ), and proliferative ( MKi-67, Top2a ) genes. DAM are defined by Clec7a and Apoe expression and further delineated by expression of Ccl3 and Trem2 . i UMAP showing enriched Nes expression within the Ccl3 DAM cluster. j-l Violin plots showing the upregulation of ( j ) Nes , k Jun , and l Fos in the CD33m + 5XFAD group relative to 5XFAD control and CD33M + 5XFAD

Article Snippet: ApoE levels were quantified in both the soluble and insoluble fractions using a Mouse Apolipoprotein E (APOE) ELISA Kit (Mybiosource).

Techniques: RNA Sequencing, Control, RNA Binding Assay, Gene Expression, Expressing